STUDY OF EXTENSIN GENE EXPRESSION AS A CANDIDATE RESPONSIBLE FOR PEA POD DEHISCENCE

The aim of this work is the study of one of the key genetic principles in domestication traits in pea – pod dehiscence by comparative analysis of wild and domesticated pea genotypes. Pod dehiscence is one of various mechanisms of seed spreading into their surroundings for the purposes of species preservation. Wild pea after maturing pods suddenly opens and fruits are scattered far and wide. This undesirable characteristic is for humans problematic because collection pea pod when touched if bursts is difficult and may cause yield loss in legumes before or during harvest. Pea is an example of crops that have undergone a process of domestication. Non-pod dehiscence, non-dormant seeds or bigger seeds are domestication traits that differentiate cultural kinds from wild species peas. There was evaluated a group of several RILs formed by crossbreeding of contrast parent genotypes of wild JI 64 Pisum sativum ssp. elatius L. and cultural pea JI92 Pisum sativum ssp. sativum L. This work was preceded by the method called MACE (Massive Analysis of cDNA Ends) to identify differences in gene expression during pod maturation. The expression of a selected gene was determined using qRT-PCR for pod dehiscence.