Brucella fluorescence polarization (FPA) detection kit

2016 
The invention relates to a brucella fluorescence polarization (FPA) detection kit. In the kit, fluorescein isothiocyanate (FITC) is adopted for marking smooth brucella O-chain lipopolysaccharide micromolecule segment (OPS) and serves as an antigen. On the basis of FPA experimental principle, the kit can be used for detecting brucellosis of various animals and simultaneously detecting IgG and IgM antigens in serum, so that the kit has more obvious advantages at sensibility aspect. According to the invention, the main reagent formula is improved and optimized, especially, the titer of positive serum is accurately marked, so that the sensitivity and specificity of the kit are effectively promoted, the problems of sensibility, repeatability, storage period, operation convenience, and the like, are solved and the time for high throughput test is greatly shortened. The invention provides a novel efficient detection method for brucellosis diagnosis in China.
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