Variance component analysis of a multi-site study for the reproducibility of multiple reaction monitoring measurements of peptides in human plasma.
2011
Background
In the Addona et al. paper (Nature Biotechnology 2009), a large-scale multi-site study was performed to quantify Multiple Reaction Monitoring (MRM) measurements of proteins spiked in human plasma. The unlabeled signature peptides derived from the seven target proteins were measured at nine different concentration levels, and their isotopic counterparts were served as the internal standards.
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