Comparison of Flavonoids from Spina Gleditsiae by Response Surface Method and Orthogonal Design

In this paper, the effects of extraction temperature, extraction pressure and dosage of entrainer on flavonoids yield were studied. The optimal process determined by response surface method was extraction temperature as 48.52°, extraction pressure as 40MPa, dosage of entrainer as 36.13mL, theoretical predictive yield as 0.791%, actual verified yield as 0.793%, and there was an interaction between the extraction temperature and dosage of entrainer. The optimum process determined by orthogonal design was extraction temperature as 50°, extraction pressure as 40MPa, dosage of entrainer as 37.5mL, and actual verified yield as 0.779%. Although the two methods obtained similar best processes, the response surface method could provide more accurate information, and the final flavonoids yield was also highest, which was worth promoting.