Green and cost-effective extraction techniques of quercetin from mixture of nutraceuticals with yield analysis via spectrophotometry and high performance liquid chromatograph methods.

BACKGROUND Extraction is the leading critical stage in the analysis of nutraceuticals. Ginkgo biloba (GB) has gained an interest because of its therapeutic usages. OBJECTIVES Development of four cost effective extraction techniques for extraction of quercetin from GB in mixture of nutraceuticals sachet. These techniques are solid phase extraction (SPE), liquid-liquid extraction (LLE), inverted dispersive liquid-liquid microextraction (IDLLME) and QuEChERS. METHODS Direct spectrophotometry was used to monitor the recovery of the standard quercetin throughout the optimization steps. HPLC-UV method of analysis was optimized to quantify the yields from the extracts present in the complicated sachets. The presented study was assessed by analytical eco-scale assessment (ESA) and National Environmental Method Index (NEMI) for greenness in comparison with literature. RESULTS Only SPE showed the best cleanup outcomes. ESA and NEMI showed an adequate greenness of proposed extraction protocol. CONCLUSION Quercetin (marker for GB) extraction from market nutraceutical sachets is considered an exemplary for analysis in quality control of nutraceuticals. Regarding the greenness results, the proposed method of extraction is grander even with adequate greenness as the extraction was one-step, in comparison with multi-steps in previously published protocols. Accordingly, it is recommended to be used in routine extraction and analysis of such kind of nutraceuticals. HIGHLIGHTS Four extraction protocols had been developed. For GB ternary sachets, proper recovery was obtained from C18 SPE. The assessment of greenness of the proposed protocol guaranteed the superiority of the presented study. Safer sorbents and chemicals favor to be used for routine extraction of nutraceuticals.