Analysis of Common Herbicides in Blood by UPLC-HRMS.

OBJECTIVES: To develop a method to screen and quantify 10 common herbicides （paraquat, diquat, glyphosate, glufosinate, cyanazine, atrazine, metazachlor, acetochlor, chlorsulfuron, and metsulfuron） in blood. METHODS: With acetonitrile-water solution [V（acetonitrile）∶V（water）=3∶1] as protein precipitant, 10 common herbicides in blood were detected using ultra-high performance liquid chromatography-high resolution mass spectrometry （UPLC-HRMS）. 方法: 以乙腈-水溶液［V（乙腈）∶V（水）=3∶1］为蛋白沉淀剂，用超高效液相色谱-高分辨质谱法（ultra-high performance liquid chromatography-high resolution mass spectrometry，UPLC-HRMS）对血液中的10种除草剂进行检测。. 结果: 10种除草剂在各自线性范围内线性关系良好（决定系数R2≥0.993），回收率为67.4%~111.9%，相对标准偏差为1.5%~10.8%，准确度为85.1%~106.1%，日内精密度为2.7%~13.5%，日间精密度为3.3%~13.3%。. 结论: 该方法操作简便、回收率高，可同时对血液中多种除草剂进行快速准确的定性筛选和定量分析。. 关键词: 法医毒理学；除草剂；超高效液相色谱-高分辨质谱；蛋白沉淀法；生物检材.