Method for manufacturing recombinant proteins using inhibitors of apoptosis.
2000
Method for producing recombinant proteins using one or more apoptosis inhibitors, comprising the steps of: (a) providing a vector comprising a gene encoding a protein of interest gene; (B) providing a host cell, Chinese hamster ovary (CHO) comprising a gene encoding a protein with negative caspase-9 dominant activity gene, wherein optionally the dominant negative gene caspase-9 has been introduced into the host cell using a vector comprising said gene; (C) transforming or transfecting the host cell with the vector of step (a); (D) providing cell culture media; (E) culturing the transformed or transfected into the cell culture medium under the conditions required for expression of the protein of interest and protein negative caspase-9 dominant activity conditions host cell; and optionally (f) recovering or purification of the protein of interest from the host cell and / or cell culture medium.
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