A Comparative Study of Effect of Essential Oil and Alcoholic Extract of Eucalyptus Leaves on Medically Important Bacterial and Fungal Isolates

Eucalyptus is a common widespread dominant tree found ecologically and economically under wide range of climatic condition. The study is focused to evaluate the medicinal property of the plant and its product which was traditionally used by the people of Asian countries like Nepal and India. The essential oil was obtained through hydro-distillation using Clevenger apparatus for 5-6 hrs and the oil percentage was found to be 2%. The alcoholic and aqueous extracts of leaves were prepared in different concentration. The antimicrobial test was studied against fungal and bacterial pathogens of plant as well as animal through agar well diffusion. The results were found to be positive on Staphylococcus aureus, Escherichia coli, Pseudomonas aeruginosa, Enterobacter aerogenes, Aspergillus niger, Aspergillus flavus, Aspergillus oryzae, Alternaria alternata, Penicillium chrysogenum and Malassezia furfur. The essential oil shows higher inhibition levels as compared to aqueous and alcoholic extr act of root and leaves. K e y w o r d s Antimicrobial activity, Hydro distillation, GC/MS, Essential oil extract, Zone of Inhibition