Evidence for microbiome-dependent chilling tolerance in Sorghum

Early season chilling stress is a major constraint on sorghum production in temperate climates. Chilling-tolerant sorghum is an active area of development, but the potential for early season microbial-enhanced chilling tolerance in sorghum has not yet been explored. In this study, we characterized traits of field-grown sorghum accessions in response to chilling and non-chilling temperatures, then we characterized the effects of chilling temperatures and microbial inoculation on sorghum accession traits in a growth chamber experiment. By comparing sorghum trait responses under chilling stress with and without soil microbial inoculation, we were able to detect a potential microbe-dependent sorghum response to chilling stress. Four sorghum genotypes show a negative response to chilling stress with vs. without microbial inoculation, while five sorghum accessions show increased shoot biomass or/ and leaf area under chilling stress when inoculated with a soil microbiome.