HPLC Determination of SudanI-IV in Food with Molecularly Imprinted Solid Phase Extraction

The molecularly imprinted polymer of Sudan red was synthesized with SudanⅠas template molecule and methacrylic acid as functional monomer,and it was used to extract SudanⅠ-Ⅳfrom food as adsorbent in solid phase extraction columa The sample was extracted with acetonitrile.and the extract obtained were purified on M1P-SPE column.SudanⅠ-Ⅳadsorbed on the imprinted polymer were eluted with ethanol-n-hexane(1 +1) mixture,which were collected and used for HPLC determinatioa It was found that the imprinted polymer had good affinity and excellent selectivity for SudanⅠ-Ⅳ.Linearity range for the determination was found between 0.5- 15 mg·L~(-1),and detection limit(3S/.V) was 4μg·kg~(-1).Using chili powder and chili sauce as matrixes,test for recovery was made at 3 concentration levels,values of recovery found were in the range of 70.3%—85.5%with RSD's(n=5) less than 6.0%.