Rational Enzyme Design Without Structural Knowledge: A Sequence-Based Approach for Effi-Cient Generation of Glycosylation Catalysts
2020
We present an enzyme engineering
approach based solely on amino-acids sequence to convert glycoside hydrolases
into transglycosylases. We demonstrate its effectiveness on enzymes form five
different glycoside hydrolase families, synthesizing various oligosaccharides
containing different α-/β-pyranosides or furanosides in one-step with high
yields.
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