The study for screening and analyzing of the differential genes of Salmonella in the VBNC state using cDNA-RDA

To study the Salmonella-specific genes in the VBNC state,and to explore the bacterial VBNC mechanisms,The method of cDNA-RDA was used to screen and analyze the Salmonella-specific gene in the VBNC state.The results showed that after three rounds subtractive hybridization,eight differential gene fragments were obtained.It was presumed that among them,there were five differential genes that may be related to the occurrence mechanism of VBNC and protein synthesis using the functional analysis of differential genes.