Bioinspired artificial small diametered vascular grafts with selective and rapid endothelialization based on amniotic membrane derived hydrogel

Clinical application of amniotic membrane (AM) in vascular reconstruction was limited by poor processability, rapid biodegradation and insufficient hemocompatibility. In this work, decellularized AM was digested to thermosensitive hydrogel and densely crosslinked in nanoscale as “enhanced” collagenous fibers. Via EDC/NHS catalysis, REDV was further grafted to simulate anticoagulant substance on natural-derived blood vessels. This modification approach endowed AM with rapid endothelialization and rare vascular restenosis. Through adjusting fixation condition, the pore size and mechanical stability of fiber network was approximate to those of natural tissue and precisely designed to fit for cells adhesion. AM was synchronously fixed by ADA and EDC/NHS, forming a “double crosslinked” stable structure significantly improved mechanical strength and resistance against enzymic degradation. Hemolytic and platelet adhesion test indicated ADA/REDV-AM could inhibit hemolysis and coagulation. It also exhibited excell...