Prevalência de Archaea em indivíduos com doença periodontal e saúde periodontal

Apesar de micro-organismos do dominio Archaea ja terem sido detectados em amostras de biofilme subgengival de individuos com periodontite, nenhum estudo comparou diretamente a associacao de Archaea com as periodontites cronica e agressiva. Assim sendo, o objetivo desse estudo foi avaliar a prevalencia do dominio Archaea em individuos com periodontite cronica generalizada (PCrG), periodontite agressiva generalizada (PAgG) e individuos com saude periodontal (SP), e correlacionar os achados microbiologicos com o aumento da profundidade de sondagem (PS). Foram selecionados 15 individuos com PCrG, 15 com PAgG e 15 com SP. Inicialmente, os individuos foram submetidos ao exame clinico-periodontal. Em seguida, amostras de biofilme subgengival foram coletadas de 9 sitios nos individuos do grupo SP e de 9 sitios (PS 7mm) nos individuos dos grupos PCrG e PAgG. Apos a extracao do DNA, as amostras foram submetidas a reacao de amplificacao do gene 16S rRNA de Archaea. A presenca de Archaeafoi detectada em 11/15 individuos do grupo PCrG, em 9/15 individuos com PAgG e 12/15 individuos com SP (p>0,05). Em relacao ao numero de amostras positivas, foram 21 no grupo SP, 39 no PAgG e 43 no PCrG observados. Nao foram observadas diferencas estatisticas entre os diferentes grupos experimentais (p>0,05) e tambem nao houve correlacao entre a presenca de Archaea e as diferentes categorias de profundidade de sondagem. Em conclusao, sugere-se ausencia de associacao entre a deteccao de Archaea e a periodontite.(AU) Archaea has been detected in subgingival biofilm samples from individuals with periodontitis, however, no studies were found to enable a direct comparison of the association of Archaea with chronic and aggressive periodontitis. Therefore, the aim of the present study was to evaluate the prevalence of the Archaea domine in individuals with generalized chronic periodontitis (GCrP), generalized aggressive periodontitis (GAgP) and periodontal healthy (PH) and correlation these finding with the increase of probing depth (PD). Fifteen individuals with GCrP, fifteen individuals with GAgP and fifteen individuals with PH were selected. The individuals were submitted a clinical periodontal examination. Subgingival biofilm samples were collected from 9 sites in the PH group and 9 sites (3 PD iU3mm, 3 PD 4-6mm and 3 PD iÝ7mm) in the 30 individuals in the GAgP and GCrP groups. After the DNA extraction, the presence of Archaea was analyzed by polymerase chair reaction (PCR). The occurrence of Archaea was detected in 11/15 individuals in the GCrP group, 9/15 individuals with GAgP and 12/15 individuals with SP (p>0.05). Among the individuals of with SP, PAgG and PCrG, 21, 39 and 43 samples were positive for Archaea, respectively. However, there were no statistical differences observed among experimental groups (p>0.05). There was no correlation between the presence of Archaea and the different probing depth categories. In conclusion, these results suggest the absence of association between the detection of Archaeaand periodontitis.