Inhibition of p75NTR in glia potentiates TrkA-mediated survival of injured retinal ganglion cells

Abstract Little is known about the molecular mechanisms that limit the ability of retinal neurons to respond to neurotrophic factor stimulation following axonal injury. In the adult retina, nerve growth factor (NGF) binds to TrkA (expressed by neurons) and p75 NTR (expressed by Muller glia), but fails to promote the survival of axotomized retinal ganglion cells (RGCs). We addressed the functional role of TrkA and p75 NTR in this lack of survival by using peptidomimetic agonistic or antagonistic ligands specific for each receptor. While administration of exogenous NGF failed to rescue axotomized RGCs, administration of selective TrkA agonists led to robust neuroprotection. Surprisingly, we found a remarkable survival of axotomized RGCs following pharmacological inhibition of p75 NTR or in p75 NTR knockout mice. Combination of NGF or TrkA agonists with p75 NTR antagonists further potentiated RGC neuroprotection in vivo , an effect that was greater than each treatment alone. NGF can therefore be neuroprotective when acting on neuronal TrkA receptors but engagement of p75 NTR on glial cells antagonizes this effect. Our data reveal a novel mechanism by which p75 NTR expressed on retinal glia can profoundly influence neuronal survival.