Study on Test-tube Flower of Tagetes patula

In order to enrich the test-tube flower materials,the system from seedlings to test-tube flowers was established for Tagetes patula in this study.The seedlings with two euphyllas on MS medium were transferred to MS medium containing various concentrations of B9 or sucrose.The results showed that B9 exhibited little influence on the plant height,but it significantly inhibited the rate of flower bud inducement.However,the plant height was obviously decreased when the sucrose concentration in MS medium increased,and it was 8.5 cm when sucrose concentration was 30 g/L,while it was only 6.1 cm when sucrose concentration increased to 80 g/L.After 50 days' culture in vitro,the florescence rate was 85% when the plants grew on the medium with 30 g/L sucrose,while it reached 100% when sucrose concentration was more than 45 g/L.MS medium with 80 g/L sucrose prolonged the flowering period by 10 days in comparison with that with 30 g/L sucrose.