Inhibition Effect of Okra Polysaccharides on Proliferation of Human Cancer Cell Lines

The anticancer bioactivity of polysaccharide has gained more and more attention in recent years.Herein,the inhibitory effect of polysaccharides extracted from okra,Abelmoschus esculentus (L.) Moench,was investigated on different human cancer cell lines,OVCAR-3,MCF-7,Hela and MCG-803 cells.Raw polysaccharide (RPS) was extracted from fresh okra fruit by ethanol precipitation and further purified by DEAE anion exchange chromatography to obtain fractions E1,E2 and E3.These cancer cells were treated with different concentrations of okra polysaccharides for 72 h and their proliferation were analyzed by MTT assay.RPS and E1 had a significant inhibition effect on the proliferation of OVCAR-3 cells in a dose-dependent manner,and the lowest survival rates were 72.30% and 52.31%,respectively.E3 had an obvious inhibition effect on MCF-7,Hela and MCG803 cells with the lowest survival rates of 63.90%,63.51% and 67.71%,respectively.Therefore,these investigations are significance of exploring and developing okra as functional foods.