In vitro cariogenic potential of Candida albicans.
2003
Summary
The adherence and dissociation of Candida albicans, C. tropicalis, Streptococcus mutans and S. sanguis to six substrates including hydroxylapatite (HAP) which exhibit various hydrophobicity, was examined by the use of a bioluminescent adenosine triphosphate (ATP) assay. Dissolution of HAP by C. albicans or S. mutans was determined spectrophotometrically by the use of o-cresolphthalein complexone. In the adherence of C. tropicalis, S. mutans and S. sanguis, the amount of adherent cells correlated with the hydrophobicity of the substrates. In contrast, the adherence of C. albicans to HAP was extraordinary high, although the adherence of the fungi also correlated with the hydrophobicity of the substrates, except for HAP. The yeasts attached to HAP was effectively removed by high concentration of either phosphate or calcium ions. The amount of calcium-release from HAP caused by C. albicans and S. mutans was 113 μg ml−1 (final pH = 3.45), and 5.4 μg ml−1 (final pH 4.81), respectively and the maximum growth of C. albicans and S. mutans was 107 cfu ml−1 and 7.4 × 1012 cfu ml−1, respectively. The results, taken together, suggest that C. albicans adhere to HAP specifically through electrostatic interaction, and that, in a much smaller number (1.0/7.4 × 105), C. albicans possesses the ability to dissolve HAP to a greater extent (approximately 20-fold) when compared with S. mutans.
Zusammenfassung
In der Arbeit wurden Adharenz von Candida albicans an Hydroxylapatit (HAP) sowie pilzbedingte HAP-Losungsvorgange untersucht. Die Adharenz von C. albicans wurde im Vergleich mit C. tropicalis, Streptococcus mutans und Streptococcus sanguis an sechs Substraten einschlieslich HAP mittels Biolumineszenz-ATP-Assay erfasst. HAP-Losung wurde spektrophotometrisch mit O-Kresolphthalein-Komplexierung bestimmt. Die Adharenzwerte korrelierten mit der Hydrophobizitat der Substrate, jedoch war die C. albicans-Adharenz an HAP ungewohnlich hoch, im Vergleich zu der von S. mutans zwanzigfach hoher. Die Adharenz an HAP konnte durch Phosphat- oder Kalziumionen beseitigt werden.
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