Long term effects of immunization against inhibin on fresh and post-thawed semen quality and sperm kinematics during low and peak breeding seasons in Beetal bucks

Abstract The present study was aimed to determine the carry-over effects of immunization against inhibin α-subunit (AIINHA) on fresh and post-thawed sperm quality during peak breeding (PBS) and low breeding seasons (LBS). Six Beetal bucks (n = 6) were either immunized against inhibin (AIINHA; n = 3) or kept untreated (CON; n = 3). Initially, the AIINHA group was treated with primary dose of inhibin α-subunit (0.50 mg) at Day 0 and followed by a booster dose 28 days later. The semen collection and cryopreservation were started five weeks post-AIINHA treatment and continued for eight consecutive weeks during LBS (n = 16 replicates; 8 in CON and 8 in AIINHA) and resumed in forthcoming eight weeks of PBS (n = 16 replicates; 8 in CON and 8 in AIINHA). Prior to cryopreservation, the fresh semen quality was assessed and afterwards, cryopreserved semen was evaluated for progressive motility, sperm kinetics, membrane, acrosomal and DNA integrities. The results indicated that AIINHA (P