Roseivivax sediminis sp. nov., a moderately halophilic bacterium isolated from salt mine sediment

A Gram-negative, facultatively anaerobic, short rod-shaped, heterotrophic bacterium, designated strain YIM D21T, was isolated from a salt mine in Yunnan province, south-west China. Strain YIM D21T formed cream–yellow colonies, was non-motile and moderately halophilic, and tolerated NaCl concentrations of 1–15 % (w/v), with optimum growth at 5–10 % (w/v). Growth occurred at 15–42 °C (optimum 28 °C) and at pH 6.5–8.5 (optimum 7.5–8.0). The respiratory quinone was ubiquinone-10 (Q-10). The polar lipids were diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylglycerol, phosphatidylcholine, three unidentified phospholipids, one unidentified aminolipid and two unidentified lipids. The major fatty acids were C18 : 1ω7c and cyclo C19 : 0ω8c and the DNA G+C content was 67.7 mol%. Phylogenetic analyses revealed that strain YIM D21T belongs to the genus Roseivivax . 16S rRNA gene sequence similarities of YIM D21T were 95.7, 95.0 and 94.8 % with the type strains of Roseivivax halodurans , Roseivivax lentus and Roseivivax halotolerans , respectively. Physiological and biochemical tests allowed phenotypic differentiation of strain YIM D21T from closely related species with validly published names. We therefore propose that this isolate represents a novel species, Roseivivax sediminis sp. nov.; the type strain is YIM D21T ( = KCTC 23444T = ACCC 10710T).