A New Steroid Ester from the Leaves of Melia azedarach

A new pregnane-type steroid, 2α,3β,16β-trihydroxy-5α-pregnane-20R-methacrylate (1), was isolated from the leaves of Melia azedarach, together with 17 known compounds. Their structures were elucidated by spectroscopic and mass-spectrometric analysis, including 1D, 2D NMR, ESI-MS, and HR-MS. Compound 1 was found to possess cytotoxicities against HepG2, K562, and SGC7901 cell lines, with IC50 values 18.6, 19.1, and 62.9 μM, respectively.