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Tatsuo Fukagawa

Graduate University for Advanced Studies
Osaka University
National Institute of Genetics

160

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9736

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A Simple Method that Combines CRISPR and AID to Quickly Generate Conditional Knockouts for Essential Genes in Various Vertebrate Cell Lines.

2022 Methods of Molecular Biology
- Kohei Nishimura
- Tatsuo Fukagawa
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Cryo‐EM structure of the CENP‐A nucleosome in complex with phosphorylated CENP‐C

2021 The EMBO Journal
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Kinetochore stretching-mediated rapid silencing of the spindle-assembly checkpoint required for failsafe chromosome segregation

2021 Current Biology
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Bridgin connects the outer kinetochore to centromeric chromatin.

2021 Nature Communications
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Transfected plasmid DNA is incorporated into the nucleus via nuclear envelope reformation at telophase

2021
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A Simple Method to Generate Super-sensitive AID (ssAID)-based Conditional Knockouts using CRISPR-based Gene Knockout in Various Vertebrate Cell Lines.

2021
- Kohei Nishimura
- Tatsuo Fukagawa
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Bub1 and CENP-U redundantly recruit Plk1 to stabilize kinetochore-microtubule attachments and ensure accurate chromosome segregation.

2021 Cell Reports
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CENP-C Phosphorylation by CDK1 in vitro

2021
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CENP-50 is required for papilloma development in the two-stage skin carcinogenesis model.

2020 Cancer Science
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A super sensitive auxin-inducible degron system with an engineered auxin-TIR1 pair

2020 bioRxiv
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