A portable 3D-printed biosensing device for rapid detection of genetically modified maize MON810

Abstract Portable 3D-printed biosensing devices have high potential as analytical platforms due to their unique characteristics. In this study, an easy-to-use biosensing device was constructed based on an event-specific recombinase polymerase amplification-lateral flow strip (RPA-LFS) method for rapid detection of genetically modified maize, MON810. We first established a rapid method for genomic DNA extraction, which achieved rapid extraction in 5 min and does not require professional equipment. The extracted DNA was then used for event-specific RPA amplification and LFS detection in a self-designed 3D-printed device that realized the detection of genetically modified maize MON810 in less than 25 min. The whole process from raw material processing to detection was rapid and easy to use. The qualitative detection limit of the method established in this study was 0.1 wt%, which meets the requirements of most countries and regions in the world for the detection of genetically modified ingredients. The results indicated that the portable 3D-printed biosensing device was sensitive and reliable with actual samples and has the potential to be applied to the rapid screening of risk factors for food safety.