C0297 Dependence of thrombin generation caused by tissue factor of microparticles on treatment with stastins

discarded. Delay before tube centrifugation was less than 2 hours. Platelet-free plasma samples were prepared using centrifugation (30 min at 1500 g) and stored at −80 °C until use. AnnV-FITC (Trevigen, USA) or AnnV-V450 (BD Horizon, USA)were used for MP labeling.MP analysiswas done using the BD LSRFortessa flow cytometer and the Gallios flow cytomer. All parameters were plotted on logarithmic scales. Annexin V-positive MP (0.5-1 μm) measurements were performed according to protocol using standard beads (Megamix, BioCytex).Each tube was analyzed during 120 sec. All data were analyzed with SPSS v.18.Data were expressed as median and Q25th75th percentiles. Correlations between MP measured by Fortessa or Gallios and between annexinV-FITC-MP and annexinV-V450-MP were determined by Spearman correlation test with a CI of 95%. Results: The following table summarizes the descriptive data about absolute values of annexinV-MP positive.