Preparation and Application of ELISA Kit for Detecting Varicella-Zoster Virus IgG

Objective To prepare ELISA kit for detecting varicella-zoster vims (VZV) IgG. Methods Inoculate VZV into diploid cells, harvest virus, purify and use as coating antigen. Label sheep anti-human IgG with HRP. The prepared kit was tested. Results TTie sensitivity of the ELISA kit was similar to that of fluorescent antibody to membrane antigen ( FAMA). Conclusion An ELISA kit for detecting VZV-IgG was successfully prepared.