Effect of G-CSF on Induction of ENA-78 and IL-8 in the Patients with Malignant Lymphoma

Granulocyte colony stimulating factor( G-CSF) restores neurtrophil count in patients with chemotherapyinduced neutropenia. G-CSF can also induce production of epithelial neutrophil activating protein-78( ENA-78) and interleukin-8( IL-8),chemotactic factors from neutrophils in vitro. This study was purposed to investigate whether this effect is also observed in vivo. 10 lymphoma patients were selected who received chemotherapy and G-CSF( nartograstim) administration. Blood was obtained before chemotherapy [Time Point 1( TP1) ],at neutropenic phase before GCSF administration( TP2),and at neutrophil recovery phase after G-CSF( TP3). ENA-78 and IL-8 mRNA in neutrophils were quantified by real-time PCR. Phagocytosis and reactive oxygen species( ROS) generation were examined by flow cytometry. The results showed that ENA-78 and IL-8 mRNA expression at TP2 increased in 5 and 8 patients,respectively. The ENA-78 mRNA expression at TP3 was increased in 3 and decreased in 6 patients,and IL-8 mRNA expression at TP3 decreased in 7 patients. G-CSF did not affect phagocytosis and normalized ROS generation in all of the patient. It is concluded that increase of ENA-78 and IL-8 expression in neutrophils is common in chemotherapy-induced neutropenic patients. G-CSF administration does not significantly increase ENA-78 and IL-8 expression.