Molecular docking and muiltple spectroscopy investigation on the binding characteristics of aloe-emodin to pepsin

Abstract In this study, the binding characteristics of aloe-emodin (AE) and pepsin were investigated via multiple spectroscopic and molecular docking methods. The Stern-Volmer quenching constant (Ksv) suggested that AE quenched the fluorescence intensity of pepsin via static quenching. The value of the stoichiometric binding number (n) suggested only a single binding site on pepsin for AE. The value of Gibbs’ free energy change (ΔG)