Bioinformatics Prediction and Comparison of Artocarpus heterophyllus BRI1 Family Members

2020 
Full-length trantogenic sequencing (PRJNA579273) of jackfruit seedling stems and leaves was performed. On this basis, biological information analysis was performed on Jackfruit ( Artocarpus heterophyllus ) BR INSENSITIVE 1 (BRI1) family members AhBRI1 , AhBRL1 and AhBRL2 to translate genes into proteins. Using online analysis tools, jackfruit, Arabidopsis (Arabidopsis Thaliana), Sichuan mulberry (Morus notabilis) and Poplar (Populus trichocarpa) were used to analyze and obtain different physicochemical properties data and the secondary and tertiary structure of the protein. The bioinformatics prediction and comparison between the BRI1 protein families of different species were obtained. The results showed that the amino acid residues were 1 195, 1 170 and 985, and the theoretical isoelectric points were 6.32, 5.77 and 7.08. The protein was weakly acidic, hydrophilic and stable. There are secretory pathway signal peptides or chloroplast transport peptides with transmembrane structure. The main components of the secondary structure are irregular coil and -helix, and the tertiary structure is spiral tubular structure. This study provides a basis for further functional studies on members of the BRI1 protein family of jackfruit.
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