Next Generation Sequencing
2021
The next generation sequencing (NGS) technology refers to non-Sanger based DNA sequencing methods which have replaced conventional sequencing methods. They have been vividly used for analyses of complete genome (whole genome sequencing), the coding exons within already reported genes (whole exome sequencing), and only coding regions of selected genes (targeted panel). In this chapter, we give an introduction of NGS technology as well as a gist of different types and applications of NGS. As advancements in NGS data analysis have opened up new therapeutic opportunities for disease diagnosis, the complementary approaches such as machine learning algorithms used in NGS are subtly dealt at the end.
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