Preparation of equine infectious anemia virus antigen for immunodiffusion test.

A simple procedure was developed for the preparation of large amounts of equine infectious anemia (EIA) virus antigen from horse leukocyte cultures or organ tissues of infected horses. This antigen showed group specificity and reacted with antiserum both in immunodiffusion and complement fixation tests. With antigen prepared from a single lot of horse leukocyte cultures, more than 6000 horses could be tested by immunodiffusion for the diagnosis of EIA.