Preliminary Study of TIP47 as a Possible New Biomarker of Cervical Dysplasia and Invasive Carcinoma

The aim of this study was to find a possible clinical use of the tail-interacting protein of 47 kDa (TIP47) and further document its expression in smear cytology, different cervical dysplasias, invasive cervical cancer and metastasis. Patients and Methods: A new polyclonal anti- TIP47 antibody was developed and used on smears and histological cervix sections of sixty women with different cytological pathologies. Serum TIP47 level of patients with cervical intraepithelial neoplasia (CIN) or carcinoma in stage IIb, IIIa, and IIIb was monitored during treatment. Results: TIP47 was expressed weakly in the dysplasias, stronger in invasive tumors and in lymph node metastasis. In patients with cervical carcinoma, the serum TIP47 level was found to be elevated; it decreased after therapy and elevated again in relapse. Conclusion: According to our results, TIP47 could be a good clinical marker for the early detection in blood of the recurrence of cervical carcinoma. An increasing number of articles reported studies of tumor suppressor genes, oncogenes, cytogenetic abnormality and human papilloma virus typing, and their role in cervical cancer. However, sufficient evidence of their use as tumor markers in clinical practice has not yet been gathered. Thus, it has become particularly urgent that thorough research be carried out on the role of oncogenes in carcinogenesis with the aim of diagnosing cervical cancer at the earliest stage possible. Evaluation of the p-STAT3 (transcription factor) expression in cervical intraepithelial neoplasias (CIN) was found to be significantly correlated with CIN lesion grade