Field evaluation of safety during gestation and horizontal Field evaluation of safety during gestation and horizontal Field evaluation of safety during gestation and horizontal Field evaluation of safety during gestation and horizontal Field evaluation of safety during gestation and horizontal

ABSTRACT..- Spilki F.R., Silva A.D., Batista H.B.C.R., Oliveira A.P., Winkelmann E., Franco A.C.,Porciuncula J.A. & Roehe P.M. 2005. [Field evaluation of safety during gestation andhorizontal spread of a recombinant differential bovine herpesvirus 1 (BoHV-1)vaccinevaccine.] Pesquisa Veterinaria Brasileira 25(1):54-58. Instituto de Pesquisa Veterinaria DesiderioFinamor, Fepagro-Saude Animal, Cx. Postal 47, Eldorado do Sul, RS 92990-000, Brazil. E-mail:proehe@ufrgs.br Bovine herpesvirus type 1 (BoHV-1) is recognized as a major cause of respiratory, reproductivedisease and abortion in cattle. Vaccination is widely applied to minimize losses induced by BoHV-1infections; however, vaccination of dams during pregnancy with modified live virus (MLV) vaccineshas been occasionally associated to abortions. We have previously reported the development of aBoHV-1 recombinant virus, constructed with basis on a Brazilian BoHV-1 (Franco et al. 2002a) fromwhich the gene coding for glycoprotein E (gE) was deleted (gE-) by genetic manipulation. Suchrecombinant has been previously evaluated in its potential as a differential vaccine (gE- vaccine)that allows differentiation between vaccinated and infected animals. Here, in the first part of thepresent study, the safety of the gE- vaccine during pregnancy was evaluated by the intramuscularinoculation of 10