Application of Allogeneic Fibroblast Cultured on Acellular Amniotic Membrane for Full-thickness Wound Healing in Rats.

Utilization of the autologous and allogeneic skin substitutes seems to be a promising treatment option. In this study, the authors used amniotic membrane covered with cultured allogenic fibroblast as a skin substitute in the treatment of acute wounds.Full-thickness wounds were created on rats' dorsum regions and treated with cultured allogenic fibroblast on an acellular amniotic membrane (AAM+F), an acellular amniotic membrane (AAM) alone, an allogenic fibroblast suspension (AFS), or normal saline as a control (C). Specimen biopsies were obtained 7 days after wounding. Quantitative wound healing parameters including the epidermal thickness, the mean number of keratinocytes, fibroblasts, and lymphocytes were assessed.All transplanted wounds exhibited significantly further contraction compared with the nontransplanted wounds. Wounds transplanted with AAM+F and AAM showed a significant increase in epidermal thickness compared to nontransplanted wounds. Wounds transplanted with AAM+F or AAM showed improved epidermal healing compared to nongrafted wounds. Furthermore, granulation of tissue formation in the AAM+F group was more organized when compared to AFS and the normal saline groups.Quantitative assessment of the full-thickness wounds showed transplantation of AAM+F and AAM better improve wound healing parameters when compared to treatment with AFS and the normal saline groups.