Multienzyme Nanoassemblies: from Rational Design to Biomedical Applications

Multienzyme nanoassemblies (MENAs) that combine the functions of several enzymes into one entity have attracted widespread research interest due to their improved enzymatic performance and great potential for diverse applications. Considerable progress has been made to design and fabricate MENAs in recent years. This review began with an introduction of the up-to-date strategies in designing MENAs, mainly including substrate channeling, compartmentalization and control of enzyme stoichiometry. The desirable properties that endowed MENAs with important applications were also discussed in detail. Then, recent advances in utilizing MENAs in the biomedical field were reviewed, with a particular focus on biosensing, tumor therapy, antioxidant and drug delivery. Finally, the challenges and perspectives for the development of versatile MENAs were summarized.