Dextran cross-linked hemoglobin-based oxygen carrier, preparation method thereof and application

The invention discloses a dextran cross-linked hemoglobin-based oxygen carrier, a preparation method thereof and an application. The preparation method includes the steps: oxidizing hydroxy on dextran 40 into an aldehyde group by sodium periodate to obtain activated dextran; protecting sulfydryl on hemoglobin by 4-PDS; crosslinking the activated dextran with the bovine hemoglobin closed by the sulfydryl; finally, de-protecting the sulfydryl to obtain the dextran cross-linked hemoglobin-based oxygen carrier. P50 of the dextran cross-linked hemoglobin-based oxygen carrier (Dex-Hb) prepared by the method can be effectively increased, oxygen carrying-releasing capacity is improved, intramolecular crosslinking of hemoglobin tetramer is realized, so that tetramer stability of HBOCs is remarkably enhanced, and physiological functions of the HBOCs are further improved.