Prognostic impact of cytogenetic evolution on the outcome of allogeneic stem cell transplantation in patients with acute myeloid leukemia in non-remission: A single institute analysis of 212 recipients

Abstract Recent progress in genetic analysis technology has helped understand the pathogenesis of acute myeloid leukemia (AML). Considering this progress, AML karyotype is still one of the most significant prognostic factors that provides risk-adapted treatment approaches. Karyotype changes during treatment have been observed at times, but their prognostic impact is sparse, especially on allogeneic stem cell transplantation (allo-SCT). Here, we retrospectively investigated the effect of chromosomal changes between diagnosis and pre-transplantation on the prognosis of allo-SCT by analyzing the outcomes of 212 consecutive patients who underwent allo-SCT for the first time at Toranomon Hospital, Tokyo, Japan, between 2008 and 2018. Cytogenetic abnormalities at diagnosis and pre-transplantation were categorized based on the 2017 European Leukemia Net (ELN) risk stratification. Genetic abnormalities such as FLT3-ITD and NPM1 were not considered in this study due to lack of genetic information in most patients. We defined cytogenetic evolution as chromosomal changes classified from lower category to higher category. Seventeen patients (8%) had cytogenetic evolution between diagnosis and pre-transplantation, and they showed a significant worse relapse rate than those who were categorized the intermediate group based on the karyotype at diagnosis (3-year CI of relapse 57.4% vs. 24.9%, p