Preparation and In Vitro Evaluation of 131I-BmK CT as a Glioma-Targeted Agent

Abstract Objective: Buthus martensi Karsch (BmK) CT, a kind of scorpion toxin peptide, was found to inhibit glioma cell proliferation in previous researches. 131I-BmK CT may have more inhibition effect and could be used as a glioma cell-targeted therapy and imaging agent. The purpose of this study was to investigate whether 131I-BmK CT could specifically conjugate with C6 glioma cell and induce glioma cell inhibition in vitro. Methods: After cloning, expression, and purification, BmK CT was labeled with 131I by indirect labeling (Bolton–Hunter method). The cell conjugation experiment was performed to investigate the connection between the reciprocal of cell conjugation rate and the reciprocal of cell count. 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-tetrazolium bromide (MTT) method and flow cytometry were used to detect the inhibition effect of BmK CT and 131I-BmK CT on glioma cell proliferation. Results: 131I-BmK CT was successfully prepared with the overall yield of 34.5%. The cell conjugation experiment...