P334 AN INFLAMMATORY EXTRACELLULAR MATRIX IS A MOLECULAR TARGET OF THE ROS SCAVENGER HIP/PAP

over a 500Hz to 64kHz frequency (f ) range, for 96h post-induction. Parallel ATP-depletion viability assays were performed (PromegaCellTiter-Glo). ECIS-8W10+ had 40 electrodes/well (averaging Z signal over hundreds of cells). ECIS-8W1E-dd arrays had a range of electrode sizes ranging from 250um down to 20um (sample specific cell populations). Results: Z -time curves showed highly sensitive dose-related response to paracetamol over the critical 24h period [Figure 1], with decreasing Z values reflecting disruption of cell–cell/cell– substrate interactions (f =4kHz); whilst at f =64kHz, Z changes were indicative of metabolic derangement and loss of membrane integrity. HepaRG ATP content mirrored Z changes. Z -spectra modelling (utilizing ECIS algorithms) supported these observations, and provided further evidence of disruption of barrier function/celladhesion parameters.