Stable Integration of Genomic Gene of Interest in Mammalian Cells Following Adeno-associated Virus Vector Mediated Gene Transfer.

An adeno associated viral vector containing erythroid enhancer(HS2), genomic β globin gene and neomycin resistance gene was constructed, then packaged into recombinant AAV virions. Southern analyses showed that recombinant provirus genome steadily integrated into erythroid cells via virus transduction. It was suggested that an adeno associated virus could mediate stable integration of genomic gene of interest in mammalian cells.