Predictive model for delivery efficiency: erythrocyte membrane-camouflaged magneto-fluorescent nanocarriers study

Delivery efficiencies of theranostic nanoparticles (NPs) based on passive tumor targeting strongly depend either on their blood circulation time or on appropriate modulations of the tumor microenvironment. Therefore, predicting the NP delivery efficiency before and after a tumor microenvironment modulation is highly desirable. Here, we present a new erythrocyte membrane-camouflaged magneto-fluorescent nanocarrier (MMFn) with long blood circulation time (92 h) and high delivery efficiency (10 %ID for Ehrlich murine tumor model). MMFns owe their magnetic and fluorescent properties to the incorporation of manganese ferrite nanoparticles (MnFe2O4 NPs) and IR-780 (a lipophilic indocyanine fluorescent dye), respectively, to their erythrocyte membrane-derived camouflage. MMFns composition, morphology, and size, as well as optical absorption, zeta potential, fluorescent, magnetic, and magneto-thermal properties, are thoroughly examined in vitro. We then present an analytical pharmacokinetic (PK) model capable of ...