Determination of Residual Catechins, Polyphenolic Contents and Antioxidant Activities of Developed Theaflavin-3,3'-Digallate Rich Black Teas
2016
This study was carried out to characterize total residual catechins and their fractions, polyphenolic
contents and antioxidant activities of black teas enriched with high levels of theaflavin-3,3’-digallate. The made teas were processed from eleven selected cultivars. A comparative study was
carried out between the processed teas and those from commercially grown Kenyan cultivars in
relation to the above chemical parameters. A correlation matrix analysis was also conducted to
find out whether a relationship existed between the antioxidant activities and the said chemical
parameters. The total residual catechins were found to range between 3.10% and 8.08%. The total
polyphenol levels varied between 19.00% and 28.90%, while the antioxidant activities of the teas
ranged from 82.70% to 91.70%. There was a significant p < 0.001 correlation between the antioxidant
activity and total polyphenols (r = 0.8948). There was also a high correlation p < 0.001
between the antioxidant activity and total catechins (r = 0.8878). Out of the four catechin fractions,
the antioxidant activity correlated most with EGCG (r = 0.8774). The total polyphenolic contents
and antioxidant activities for most of the cultivars were comparable to those of the green tea reference
standard. From the figures obtained, it can be concluded that the most of the newly developed
black teas of the selected cultivars have higher quality and enhanced antioxidant activities
and that they can be recommended for commercial production.
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