Establishment of Sichuan potato virus detection by RT-PCR.

Based on the nucleotide sequence of the coat protein(CP) gene of the five main potato virus in our country,five pairs of DNA primers were designed.The total RNA was directly extracted from virus-infected potato leaves.By the reverse transcription and polymerase chain reaction(RT-PCR),and a specific fragment with expected length was obtained.The detection of potato virus by RT-PCR was established and applied to the Sichuan potato virus detection.A new method of the Sichuan potato virus detection was established in molecular level.