Implications of high expression of miR17-92 cluster in ovarian cancer (OvCa) tissue found in long term survivors.

e12561 Background: Specific tissue miRNAs play an important role in tissue identity, differentiation and function. MiR-17-92 cluster is deleted in 20% of ovarian cancers. This is essential in embryonal and immune system development. Long term survival in OvCa may correlate with miR expression. We set out to identify levels of miR17-92 in OvCa tissue that correlated with the patients’ long-term outcomes. Methods: Tissue miRNAs were collected at surgery - tumor and benign (control) ovary from consented women. Taqman OpenArray panel was used to simultaneously run 745 microRNA using Biosystems Openarray RtPCR; 2-tailed t-test was used for all 2-sample comparison and ANOVA followed by Benjamin-Hochberg method for multiple testing to limit the false discovery rate (FDR) at the 5% level. Target scan context+ scores identified mRNAs regulated by miR17-92. Reactome constructed deregulated pathways. Results: Mean age at surgery 65 (range 51-78), 11 race matched women – mean age of 58 (range 25-67); 21 patients died...