Determination of phenols in water using liquid phase microextraction with back extraction combined with high-performance liquid chromatography

Abstract Liquid phase microextraction with back extraction (LPME/BE) combined with high-performance liquid chromatography (HPLC) was studied for the determination of a variety of phenols in water samples. The target compounds were extracted from 2-ml aqueous sample adjusted to pH 1 (donor solution) through a microliter-size organic solvent phase (400-μl n -hexane), confined inside a small PTFE ring, and finally into a 1-μl basic aqueous acceptor microdrop suspended in the aforementioned solvent phase from the tip of a microsyringe needle. After extracting for a prescribed time, the microdrop was taken back into the syringe and directly injected into an HPLC for detection. Factors relevant to the extraction procedure were studied. At the optimized extraction conditions, a large enrichment factor (more than 100-fold) can be achieved for most of the phenols within 35 min. The detection limit range was 0.5–2.5 μg/l for different analytes in aqueous samples. The results demonstrate the suitability of the LPME/BE approach to the analysis of polar compounds in aqueous samples.