Quantum-dot-based immunofluorescent imaging of Cancer Marker P53 Detection in Breast Cancer

The study is to design a biomarker based on hydrophilic quantum dots(QDs) can be bound to the P53 protein for diagnoses of breast cancer (BC).To achieve the purpose, we uses the water-soluble quantum (QDs) AgInS2/ZnS connected with P53 monoclonal antibody to design a novel kind of biomarker as a new detection of breast cancer. Significantly, this targeted quantum dots not only have good target recognition ability, but also have low toxicity and strong light stability. Moreover, confocal laser scanning microscopy (CLSM) showed that the localized fluorescence would be detected around the nucleus of human breast cancer cell MCF7 (high expression of P53) compared with that of T47D (low expression of P53). In summary, we believe that this QDs probe will provide a broad platform for clinical diagnosis and treatment of breast cancer and in vitro studies in future.