Characterization and stability of a bioactivated alumina nanomembrane for application in flow devices

Abstract Surface modification of alumina nanomembrane (ANP) by theophylline derivative Theo 1 by dip-coating was successfully achieved. The hybrid material was characterized by SEM, XPS and confocal microscopy. The presence of Theo 1 on the ANP surface was established from salt diffusion and membrane potentials measurements. Changes in the electrical character and transport parameters of the modified sample, associated to Theo 1 deposition, were established. Stability of the material under diffusive flow was also ascertained. Moreover, as a result of Theo 1 bioactivation, streptavidin linkage to the ANP/Theo 1 -modified membrane was obtained, which is an indication of streptavidin capture.