Experimental study on changes of serum total IgE,IL - 4 and IL - 5 in mice with Trichinella spiralis induced allergic asthma

Objective To study the effect of Trichinella spiralis infection on total IgE,IL-4 and IL-5 in serum of the mice with allergic asthma.Methods Twenty female BALB / c mice were randomly divided into four groups of five mice each,including Ⅰ as control group,Ⅱ as allergic asthma group,Ⅲ as asthma followed by Trichinella spiralis infection,Ⅳ as Trichinella spiralis infected group.Firstly,the animals in group Ⅲ and Ⅳ were infected with 200-300 Trichinella spiralis muscle larvae.Twenty-eight days later,ovalbumin(OVA) was used to induce the allergic asthma for mice in group Ⅱ and Ⅲ,in order to establish asthma animal model.After 56 days,all mice were sacrificed,levels of TIgE,IL-4 and IL-5 in serum were measured by ELISA.Results The TIgE levels in groupⅠ,Ⅱ,Ⅲ and Ⅳ were(61.79 ± 25.79) ng / ml,(437.08 ± 75.68) ng / ml,(251.64 ± 107.27) ng / ml and(446.12 ± 74.32) ng / ml,respectively.The IL-4 levels in groupⅠ,Ⅱ,Ⅲ and Ⅳ were(136.49 ± 31.17) pg / L,(209.80 ± 21.57) pg / L,(162.79 ±17.69) pg/L and(182.71 ±12.08) pg/L,respectively.IL-5 levels in groupⅠ,Ⅱ,Ⅲ and Ⅳ were(7.31 ±1.86) ng/ L,(16.00 ± 2.29) ng / L,(9.24 ± 1.73) ng / L and(11.41 ± 2.11) ng / L,respectively.Compared with group Ⅱ,the TIgE、IL-4 and IL-5 levels in group Ⅲ decreased(P 0.05).Conclusions Trichinella spiralis infection can inhibit expression of the TIgE,IL-4 and IL-5 in serum of mice with allergic asthma.