Red blood cell damage during experimental prolonged perfusion with membrane oxygenation using fresh human blood

The effects of prolonged perfusion of oxygenated blood have previously been studied with respect to haemolysis and cell morphology. The aim of this study was to examine the effect of mechanical trauma on the microrheology of red blood cells during experimental prolonged perfusion with membrane oxygenation (PPMO). Red blood cell damage was assessed by blood rheological parameters using a St George's filtrometer. Red blood cell filtration rate (RFR, μl/s), clogging rate (RBC-CR, 102%/ml), clogging particle (RBC-CP, 106/ml), mean corpuscular volume (MCV) and haematocrit (Hct) were analysed at the start of PPMO and after 24, 48 and 72 hours. RFR values were 81.3 ± 3.7 at the start, 79.2 ± 7.6 (24 h, p < 0.01), 42.3 ± 8.4 (48 h, p < 0.001) and 25.1 ± 7.0 (72 h, p < 0.001). The mean RBC-CR was 2.45 ± 0.53 at the start; this increased to 3.58 ± 0.9, 6.62 ± 0.92 and then reduced to 4.77 ± 1.39 at 24 (p < 0.0001), 48 (p < 0.0001) and 72 (p < 0.02) hours respectively. Mean RBC-CP at the start was 3.29 ± 0.55; this ...