Antioxidant and anti-aging activities of the polysaccharide TLH-3 from Tricholoma lobayense

Abstract Polysaccharides from edible fungi usually exhibit many bioactivities. Our previous studies found that polysaccharide TLH-3 extracted from Tricholoma lobayense possessed noticeable antioxidant activity. To further explore its biological activities, the antioxidant and anti-aging activities of TLH-3 were evaluated in vitro and in vivo . The results of antioxidant activity in vitro showed that TLH-3 could enhance the cell viability, reduce the production of reactive oxygen species (ROS) and inhibit oxidative damage induced by tert -butylhydroperoxide ( t -BHP) in human embryonic lung fibroblasts (HELF) cells. The anti-aging capability was measured in d -galactose ( d -gal)-induced aged mice model, and the experimental data showed that TLH-3 significantly inhibited the formation of malondialdehyde (MDA) and raised the activities of superoxide dismutase (SOD) and catalase (CAT) in mice liver and serum ( p