Influence of wound fluid on chemotherapy sensitivity in primary breast cancer cells

// Yingchao Zhang 1, * , Dong Yan 2, * , Hao Zhang 3 , Xunyan Ou 3 , Zuowei Zhao 3 , Dan Wang 3 , Caigang Liu 3 1 Department of Breast Surgery, The Second Hospital of JiLin University, Changchun, 130021, China 2 Department of Pediatrics, The Second Hospital of Dalian Medical University, Dalian, 114006, China 3 Breast Disease and Reconstruction Center, Breast Cancer Key Laboratory of Dalian, The Second Hospital of Dalian Medical University, Dalian, 114006, China * Co-first authors Correspondence to: Caigang Liu, email: angel-s205@163.com Keywords: breast cancer, wound fluid, chemotherapy, drug resistance Received: April 10, 2016      Accepted: August 10, 2016      Published: August 17, 2016 ABSTRACT Objective: To investigate the effect of WF on chemotherapy sensitivities of primary breast cancer cells from breast cancer patients by using CD-DST. Results: In general, the WF-treated cells showed remarkable increase in survival rates as compared to the control cells cultured without WF among different anticancer drug subgroups. This trend was generally observed in all the tumor cells from the premenopausal, postmenopausal, T2, N0, N1, luminal B, and TN patients. Methods: The sensitivities of WF-treated primary breast cancer cells, from 21 patients who underwent a radical resection for breast cancer from September 2014 to July 2015, to anticancer drugs: EPI, CDDP, DOC, VNR, 5-FU+LV, and PAC, were obtained using CD-DST. The survival rates of the breast cancer cells were recorded and used to gauge the chemotherapeutic effect. Conclusions: Surgery-induced WF promotes the drug resistance of primary breast cancer cells to chemotherapy, suggesting that surgery may have adverse effects on breast cancer patients. More studies are needed to investigate the key factors in WF that enhance the susceptibility to chemotherapy drugs.